CD3 antibody

Catalog No. ABIN120762

This anti-CD3 antibody is a Mouse Monoclonal antibody detecting CD3 in FACS, IP and Func. Suitable for Human. This Primary Antibody has been cited in 8+ publications.

Quick Overview for CD3 antibody (ABIN120762)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: MEM-92

Product Details anti-CD3 Antibody

Specificity: The antibody MEM-92 reacts with epsilon chain of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.

Purification: Thiophilic adsorption-affinity chromatography

Purity: > 95 % (by SDS-PAGE).

Immunogen: Human peripheral blood lymphocytes

Isotype: IgM

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Functional Application: The antibody in solution induces early responses of T cell activation (tyrosine phosphorylation, calcium elevation, Erk activation and expression of activation antigens), but it is unable to induce T cell proliferation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. Do not freeze.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-CD3 antibody (ABIN120762)

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, (2007) (PubMed).

Batista, Millán, Mittelbrunn, Sánchez-Madrid, Alonso: "Recruitment of transferrin receptor to immunological synapse in response to TCR engagement." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6709-14, (2004) (PubMed).

Haeryfar, Hoskin: "Thy-1: more than a mouse pan-T cell marker." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3581-8, (2004) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, (1997) (PubMed).

Stefanová, Saville, Peters, Cleghorn, Schwartz, Venzon, Weinhold, Jack, Bartholomew, Blattner, Yarchoan, Bolen, Horak: "HIV infection--induced posttranslational modification of T cell signaling molecules associated with disease progression." in: The Journal of clinical investigation, Vol. 98, Issue 6, pp. 1290-7, (1996) (PubMed).

Soucek, Hilgert, Budová, Lindnerová: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, (1994) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T-cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway